绪论 单元测试

1、多选题：
Biochemistry can be divided into three principal areas, that is：（ ）
选项：
A:metabolism, the totality of chemical reactions that occur in living matter
B:morphology change
C:the structural chemistry of the components of living matter and relationships of biological function to chemical structure
D:genetic biochemistry, the chemistry of processes and substances that store and transmit biological information
E:study of structure of the Human Body
答案: 【metabolism, the totality of chemical reactions that occur in living matter;
the structural chemistry of the components of living matter and relationships of biological function to chemical structure;
genetic biochemistry, the chemistry of processes and substances that store and transmit biological information】

第一章 单元测试

1、单选题：
The nitrogen content of one protein sample is 0.5g，and then the protein content of it is ：（ ）
选项：
A:12.5g
B:1.565g
C:1.25g
D:3.125g
E:6.25g
答案: 【3.125g】

2、单选题：
Which of the followings is basic AA? （ ）
选项：
A:Pro
B:Tyr
C:Glu
D:Lys
E:Met
答案: 【Lys】

3、单选题：
Which of the following about α-helix is not correct：（ ）
选项：
A:the helix is clockwise
B:one pitch is 0.54nm
C:the helix is right handed
D:3.6 residues per circle
E:the peptide bonds are the major bonds to stabilize the helix
答案: 【the peptide bonds are the major bonds to stabilize the helix】

4、单选题：
One AA( pI=5.6 ) will be negatively charged in a solution （ ）?
选项：
A:pH8.6
B:pH5.6
C:pH4.7
D:pH2.6
E:pH5.0
答案: 【pH8.6】

5、单选题：
Which of the following about protein denaturaion is not correct?（ ）
选项：
A:the physico-chemical property of protein changed
B:precipitated protein not be sure to be denatured(C)
C:denatured protein must precipitate
D:The spatial structure of protein are destroied
E:the secondary bond of protein are broken
答案: 【denatured protein must precipitate】

6、单选题：
All the common proein absorb light at a wavelength of UV close to （ ） nm?
选项：
A:260nm
B:280nm
C:340nm
D:300nm
E:200nm
答案: 【280nm】

7、单选题：
The amino acid found as constituents of mammalian proteins are all ( ) except for glycine?（ ）
选项：
A:L-β-AA
B:L，D-α-AA
C:D-α-AA
D:D-β-AA
E:L-α-AA
答案: 【L-α-AA】

8、单选题：
Which of the following about protein secondary structure is not correct? （ ）
选项：
A:amino acid sequence of protein
B:it did not involve in the conformation of side chain of amnio acid
C:it is local spatial structure of one certain part of peptide chain in protein
D:it is the relative spatial position of skeleton atom on protein main chain
E:it is hydrogen bond that maintain the stability of protein secondary structure
答案: 【amino acid sequence of protein】

9、单选题：
Which of the following about quaternary structue is correct? （ ）
选项：
A:the stability of protein quaternary structure was maintained by disulfide bond
B:the subunit were polymerized by noncovalent bond
C:all the protein have quaternary structure
D:quaternary structure was essential condition for maintaining the protein biological activity
E:quaternary structure of protein would not sure to be destoried
答案: 【the subunit were polymerized by noncovalent bond】

10、单选题：
α helix of protein structure belongs to（ ）
选项：
A:secondary structure
B:quaternary structure
C:motif
D:tertiary structure
E:domain
答案: 【secondary structure】

第二章 单元测试

1、单选题：
5’-end of the majority of mRNA in eukaryotes has （ ）
选项：
A:stop codon
B:cap sequence
C:initiation codon
D:poly (A) tail
E:CCA-OH
答案: 【cap sequence】

2、单选题：
Which statement is NOT true for tRNA? （ ）
选项：
A:Various tRNA are present in the cell.
B:The secondary structure of tRNA is cloverleaf structure.
C:tRNA contains rare bases.
D:tRNA is usually composed of 70-90 nucleotides.
E:Anticodon loop contains the anticodon that is composed of three bases CCA.
答案: 【Anticodon loop contains the anticodon that is composed of three bases CCA.】

3、单选题：
Which statement is NOT true for RNA? （ ）
选项：
A:rRNA is the place for protein synthesis.
B:It is usually single-stranded molecule.
C:mRNA contains the genetic codon.
D:tRNA contains more rare bases than other RNAs.
E:RNA contains regional double-stranded structure.
答案: 【rRNA is the place for protein synthesis.】

4、单选题：
Which statement is true for Tm? （ ）
选项：
A:Higher AT content, higher Tm
B:is usually RNA
C:Higher GC content，higher Tm.
D:Higher purity of nucleic acid, larger range of Tm
E:Smaller nucleic acid, larger range of Tm.
F:The nucleic acid with higher
答案: 【Higher GC content，higher Tm.】

5、单选题：
The secondary structure of DNA is （ ）
选项：
A:double-stranded helix
B:regional double-stranded helix
C:polyA sequence
D:supercoil
E:inverted L shape
答案: 【double-stranded helix】

6、单选题：
The tertiary structure of tRNA is （ ）
选项：
A:regional double-stranded helix
B:supercoil
C:polyA sequence
D:inverted L shape
E:double-stranded helix
答案: 【inverted L shape】

7、单选题：
3'-end of mRNA has （ ）
选项：
A:supercoil
B:inverted L shape
C:double-stranded helix
D:regional double-stranded helix
E:polyA sequence
答案: 【polyA sequence】

8、单选题：
The RNA that can carry amino acid and transfer it to ribosome is （ ）
选项：
A:siRNA
B:tRNA
C:mRNA
D:rRNA
E:hnRNA
答案: 【tRNA】

9、单选题：
The template of protein synthesis is （ ）
选项：
A:siRNA
B:tRNA
C:hnRNA
D:mRNA
E:rRNA
答案: 【mRNA】

10、单选题：
DNA can be separated into two single chains during heating （ ）
选项：
A:DNA denaturation
B:DNA renaturation
C:Hydrolysis of nucleic acid
D:Dissociation of nucleic acid
E:Hybridization
答案: 【DNA denaturation】

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